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Novel Validated Ultra Violet Spectroscopic Methods for the Determination Of Nordihydroguaiaretic Acid in Dietary Supplements.

Ramadoss Karthikeyan, P Srinivasa Babu, and D Monica Paulin

A simple, rapid, accurate and precise,Spectrophotometric method was developed for determination of Nordihydroguaiaretic acid. In this method Nordihydroguaiaretic acid shows absorbance maximum at different wavelength in different solvents, so absorbance was measured and the same wavelengths used for the estimation of Nordihydroguaiaretic acid in respective solvents. Nordihydroguaiaretic acid obeys the beer lamberts’ law. Method is validated according to ICH guideline and carried out for analysis of Nordihydroguaiaretic acid.