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Studies on Optimization of Process Parameters for Nattokinase Production by Bacillus subtilis NCIM 2724 and Purification by Liquid-Liquid Extraction

Peddapalli Siva Rasagnya And Meena Vangalapati

Nattokinase (NK) is one such thrombolytic enzyme. Nattokinase is an enzyme with strong fibrinolytic activity that can be used for preventing thrombolytic diseases. It is a serine protease class of enzyme. Nattokinase finds its application in medical industry widely. It provides health benefits like cure of hemorrhoids, Diabetes, Muscle spasms, poor healing, chronic inflammation, help improve blood clotting mechanism, improve blood circulation, blood viscosity etc,. In the present study, fermented soya bean powder is used as substrate for nattokinase production and the optimized parameters like incubation time-24hrs, inoculum volume-1ml, inoculum age-4th day and pH-7.5 that effect the production are optimized and the crude enzyme obtained was purified by using 2-phase liquid extraction technique