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Perspectives on Elevated Ploidy in Regenerants from Plant Tissue Culture and Transformation

Jenny W Jing, Thomas M Davis, Qian Zhang

Elevated ploidy is a common phenomenon among plants regenerated from tissue culture. Contributors to this effect include cells of elevated ploidy present within normally diploid or polyploidy explant tissues, endoreduplication by individual cultured cells, and effects of in vitro environmental factors on cell division. Because polyploidization can result in morphological changes and thus, different adaptabilities, the determination of ploidy in transgenic regenerants plants is recommended when evaluating phenotypes and conducting mutant screens subsequent to genetic transformation.