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Mathematical Modelling Approach for Correlating Spectrophotometric Absorbance with Dissolved Oxygen

Vandana Sharma, Urvasini Singh, Ravi Kumar Malviya, Menaka Khicher, Jayashri Vajpai and Sunita Kumbhat

A mathematical model has been developed in this paper for determination of dissolved oxygen (DO) in water samples by measuring the absorbance of monochromatic light by the sample. Determination of dissolved oxygen is an important parameter in the study of water quality. Winkler’s method, based on titrations, is well established as the standard method for the determination of the DO. Experimentation was carried out to measure the dissolved oxygen and the absorbance of the water samples. Mathematical modelling has been carried out for determining DO values as a function of the absorbance observed for the samples. The correlation between absorbance of monochromatic light by the sample and DO values was determined. This correlation coefficient was found using curve fitting tools in the MATLAB software. Finally, the DO values hence obtained were compared with and found to be in close agreement with those obtained by Winkler’s method.