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Isolation and Characterization of Steroidal Glycoside from Chloroform Extract of the Stem Bark of Moringa Pterygosperma Gaertn

Rohit Kumar Bargah, Chinmoyee Das

General phytochemical screening of the chloroform extract of the stem bark of Moringa pterygosperma (Moringaceae) revealed the presence of steroids, alkaloids, terpenoids, phenolic compounds, fatty acids, saponins. The aim of the study is to identify and characterize the bioactive constituents from the stem bark of the plant. It has wide folk medicinal use. Isolation of the compound was carried out by chromatographic techniques; isolated compound was purified by chloroform. The isolation and purification afforded white crystalline powder which was subjected to physical, chemical and different spectral analytical methods. The spectral data of UV-Vis, FT-IR, 1H-NMR, 13 C NMR and GC-MS of the isolated compound is in support of presence of steroid Nucleus. The compound has been characterized as β-Sitosterol 3-O-β -D- galactopyranoside.