Sheeba.E., S.Palanivel and S.Parvathi
Different concentrations and combinations of hormones were used in MS medium to observe callus induction and then plant regeneration using leaf as explant. The rate of callus formation varied in different treatments used. The highest amount of callus (80.67%) was produced on MS medium containing 3.0 mg/l NAA from leaf after 20 days. The highest fresh weight of callus was obtained from 2.0 mg/l IAA and 0.5 mg/l BAP. The highest percentage of regeneration (46.28%) was recorded in MS media containing 2.0 mg/l BAP + 0.25 mg/l IAA from leaf after 42 days. The protocol in the study might be useful for the production of disease free and healthy plant materials and also it would be useful for genetic transformation and secondary metabolite production of Physalis minima using biotechnological approach
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